Endocrine Abstracts

Background: Cushing’s syndrome (CS) is associated with high morbidity and presents high interindividual variability. Easily measurable biomarkers, in addition to the hormone assays currently used for diagnosis, could better quantify the individual biological impact of glucocorticoids. The aim of this study is to identify such biomarkers through the analysis of whole blood transcriptome.Methods: Whole blood transcriptome was evaluated in 57 samples (...

Corticotroph Pituitary Neuroendocrine Tumours (Co-PitNETs) show significant differences in cortisol secretion, responses to existing therapies and gene expression andstill pose a challenge for patient management.Aim: to explore possible novel therapeutic targets in Co-PitNETs (functioning and silent) through transcriptome analysis (RNA sequencing) and immunohistochemistry. Methods: Transcriptomic data from a previously reported dat...

PAM encodes a multifunctional protein recognized as an important regulator of hormone amidation and secretion. Since PAM germline mutations have been recently identified in patients with PitNETs, it has been proposed as a candidate gene associated with pituitary hypersecretion.Aim: To characterize PAM expression in a large cohort of PitNETs through immunohistochemistry and transcriptome analysis (RNA sequencing).M...

An initial multi-omics analysis of PitNETs has refined histological classifications, and could improve diagnostic and prognostic assessment (Neou, Cancer Cell 2020). Of all omics, transcriptome best discriminates between these different classes. This molecular classification has been built upon frozen samples, which are difficult to use in routine clinical practice.Aim: Demonstrate the feasibility of measuring the transcriptome in Formalin-Fixed Paraffin...

Introduction: Adrenal steroidogenesis is alterated in adrenocortical tumors (ACT), leading frequently to abnormal steroid secretion. The development of steroid assays by mass spectrometry (MS) has greatly advanced the characterization of these alterations. However, steroid profiles are classically performed in blood and urine, providing a limited insight into adrenal steroidogenesis, altered by the mixing with gonadal steroids and by the steroid peripheral catabolism. The obje...

Background: Bulk genomic studies have identified distinct molecular classes of adrenocortical tumors (ACT). Transcriptome profiles separate benign ACT (“C2” cluster) from carcinomas (ACC) and identify two groups of ACC, “C1A” (“steroid” and “proliferation” signatures) and “C1B” (“immune” signature), of poor and better prognosis respectively. However, these signatures were characterized at the tissue level (“bulk&...